Infectious bronchitis virus: Evidence for recombination within the Massachusetts serotype

detection of avian infectious bronchitis virus and massachusetts serotype by rt-pcr

the aim of the present study was using rt-pcr for the diagnosis of avian infectious bronchitis virus and massachusetts serotype in tissue samples. optimization of a molecular diagnostic method for the detection of avian bronchitis virus and identification of massachusetts serotype was investigated. in order to detect infectious bronchitis virus (ibv) in tissue samples, an rt-pcr was optimized. ...

Experimental histopathologic study of the lesions induced by serotype 793/B (4/91) infectious bronchitis virus

The study was conducted to determine the type, frequency and severity of gross, histopathologic changes, effect of route of infection and also, tissue tropism of serotype 793/B (4/91) infectious bronchitis virus. 20 day-old specific pathogen free chicks were inoculated intra-tracheally and intra-orally with 793/B serotype and lesions studied histopathologically at interval up to 10 days post-in...

Molecular Cloning and Expression of Nucleocapsid Gene of Chicken Infectious Bronchitis Virus Strain Massachusetts H120

Chicken infectious bronchitis virus (IBV) causes severe respiratory and renal dysfunction in chickens. In the present study, we used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the nucleocapsid (N) protein gene of strain Massachusetts (Mass) H120 of IBV that is commonly used in the vaccine production in Iran. The PCR product with the expected size of 1.2 kb was cloned in...

Serotype Identification of Recent Iranian Isolates of Infectious Bronchitis Virus by Type-Specific Multiplex RT-PCR

Recombination in Avian Gamma-Coronavirus Infectious Bronchitis Virus

Recombination in the family Coronaviridae has been well documented and is thought to be a contributing factor in the emergence and evolution of different coronaviral genotypes as well as different species of coronavirus. However, there are limited data available on the frequency and extent of recombination in coronaviruses in nature and particularly for the avian gamma-coronaviruses where only ...